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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is observed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential position in marketing mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are explained. Two supplemental splice variants were being documented but thorough nucleotide sequence facts is not really nonetheless out there.

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Blocking peptides are peptides that bind particularly to your concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.

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This guidebook outlines further more attainable optimization of mobile and immunoassay parameters to make certain the best possible outcomes are...

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This guide outlines even more possible optimization of cellular and immunoassay parameters to guarantee the best possible final results are...

Antibodies sure to the blocking peptide now not bind on the epitope around the focus on protein. By evaluating the staining from your blocked antibody as opposed to the antibody alone, one can see which staining is distinct.

Concentrate on Info Cyclin E belongs on the really conserved cyclin loved ones, whose associates are characterised by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins operate as regulators of CDK kinases. Distinctive cyclins exhibit distinct expression and degradation patterns which contribute towards the temporal coordination of every mitotic celebration. Cyclin E varieties a fancy with and functions as being a regulatory subunit of CDK2, whose exercise is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S period boundary which is degraded as cells progress via S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor get more info activation by equally antibodies...

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